Enzymes are proteins that act as biocatalysts and help increase the rate of biochemical reactions in living organisms. These are important for biological processes such as digestion and liver function. An excess or lack of a certain enzyme can lead to health problems. Enzymes are also used in multiple biotechnological industrial processes after being extracted from cells. They are used extensively in industries such as food processing industry, pharmaceutical industry, dairy industry, biofuel industry, and brewing industry. Proteases, amylases and lipases are commonly used enzymes in biological washing powders and various cleaning products. In food processing industry, amylase enzyme is used in the production of sweetening agents. Enzymes also play a fundamental role in analytical devices and assays that have forensic, clinical and environmental applications. This book includes some of the vital pieces of works being conducted across the world, on various topics related to enzyme biotechnology. It is a valuable compilation of topics, ranging from the basic to the most complex advancements in this field. The book will serve as a valuable source of reference for graduate and postgraduate students.